| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061405 |
| Chromosome: | chromosome_17 |
| Location: | 514760 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g699500 | TTL8,TTLL9,FAP267 | Tubulin tyrosine ligase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGCGTGGCTGCATGTGCCATGCGTCTCGC |
| Internal bar code: | TCGGGATGCGCGCCCGTAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAAAGCCGCTGGAATAG |
| Suggested primer 2: | ATCGTGGTTTACCGAGATGC |