| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061417 |
| Chromosome: | chromosome 7 |
| Location: | 5758441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352950 | (1 of 1) IPR003395//IPR027417 - RecF/RecN/SMC, N-terminal // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCTTTCCCCGTGCCCCCCTCTCGTGG |
| Internal bar code: | GTTTCAAGACCGTGATAGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 99.0881 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGCGTGTGTGTTAGGTT |
| Suggested primer 2: | GGTTAAGGTGCGTGTGTGTG |