| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061422 |
| Chromosome: | chromosome 6 |
| Location: | 3614992 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278125 | (1 of 1) PF09379 - FERM N-terminal domain (FERM_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCCCCGTTTGGCCTCGCCGCCAGGTT |
| Internal bar code: | CGGGGTAAGGCATGTACCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 84.3521 |
| LEAP-Seq n confirming: | 6458 |
| LEAP-Seq n nonconfirming: | 1198 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATGTCGTTGATGTGCGT |
| Suggested primer 2: | TTCACTCTGTTTGAGTGCCG |