| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061433 |
| Chromosome: | chromosome 10 |
| Location: | 1689277 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430150 | REP27,LPA1 | (1 of 1) PF11998//PF13414 - Protein of unknown function (DUF3493) (DUF3493) // TPR repeat (TPR_11); Low Photosystem II Accumulation 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACAGCCTCCTGTGTACTGCCTAGGCGC |
| Internal bar code: | TAGACCTTGCCCTATGCTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 98.6726 |
| LEAP-Seq n confirming: | 892 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCGAATGTGGCAATGTG |
| Suggested primer 2: | CCCAACTGCTACATCCAGGT |