Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.061439 |
Chromosome: | chromosome_12 |
Location: | 5245216 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g528250 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATGCGACGTTTCACCAACTGGGATCACCC |
Internal bar code: | CCGCCAGTCGGCCAGACGTCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1065 |
LEAP-Seq percent confirming: | 99.4874 |
LEAP-Seq n confirming: | 6793 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCTGCCCATTATCTTGCAC |
Suggested primer 2: | GATCTCAATGGGGCTTTTCA |