| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061440 |
| Chromosome: | chromosome 16 |
| Location: | 4455530 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670850 | (1 of 1) PTHR22975:SF2 - ECHINUS SPLICE FORM 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTTAAATCATCGACGGTGTTGCAGCC |
| Internal bar code: | CCGGCCCTGGCAGCGTACGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 99.475 |
| LEAP-Seq n confirming: | 3221 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTCAAAAGAGGACAAAC |
| Suggested primer 2: | TACCTGGTGCGTGTGTTTGT |