| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061444 |
| Chromosome: | chromosome_7 |
| Location: | 3989184 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g339850 | antisense | intron|outside_mRNA | ||
| Cre07.g339876 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGTCGCGTTTGACAGTGTGATCCGATGTT |
| Internal bar code: | CGTGATCTAGGTAGGCGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 95.159 |
| LEAP-Seq n confirming: | 1317 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTAACTGGGGCCGTGTA |
| Suggested primer 2: | CAAGTGCTTAATAAGGCGGC |