| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061448 |
| Chromosome: | chromosome 8 |
| Location: | 3539299 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375400 | GSM1 | (1 of 1) PTHR11850//PTHR11850:SF102 - HOMEOBOX PROTEIN TRANSCRIPTION FACTORS // SUBFAMILY NOT NAMED; Gamete-specific minus 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGTCCAGTTCTCCTTCCCAGTAGACCT |
| Internal bar code: | GGGAGCTGGAGGGAGGAAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 99.7408 |
| LEAP-Seq n confirming: | 1924 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGATGCAATGCGATGTT |
| Suggested primer 2: | ATCATCACCGACCCAACAAT |