| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061459 |
| Chromosome: | chromosome 2 |
| Location: | 1498659 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084150 | (1 of 60) IPR001611 - Leucine-rich repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAAGCCCAGGAGTTGCCAGGAGGGCTC |
| Internal bar code: | GGCACCTCGTATCGACTCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 739 |
| LEAP-Seq percent confirming: | 65.4804 |
| LEAP-Seq n confirming: | 184 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCTGTTTTGGAACCTGT |
| Suggested primer 2: | TGGTTGTGACGTGAGAGAGC |