| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061462 |
| Chromosome: | chromosome 3 |
| Location: | 6646860 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197050 | HTV2 | (1 of 35) K11253 - histone H3 (H3); Histone H3 variant | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAACCACGGGTCGAAGGGTGCTGCGCCAT |
| Internal bar code: | TCGCACCGTTAAATCCTTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 99.7356 |
| LEAP-Seq n confirming: | 1509 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACAACGGAGCTGCTCAT |
| Suggested primer 2: | CCCGCATCACTACCGTACTT |