| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061464 |
| Chromosome: | chromosome 14 |
| Location: | 3338269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629960 | (1 of 2) 2.3.2.15 - Glutathione gamma-glutamylcysteinyltransferase / Phytochelatin synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGACTGCACAGTTGCGGTCCCGCGACT |
| Internal bar code: | CTGGCCGGAAAGCCGTGCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 89.1473 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCACTTCTCTGTACCAA |
| Suggested primer 2: | ACTCCAACAACGACACTCCC |