Insertion junction: LMJ.RY0402.061473_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.061473
Chromosome:chromosome 3
Location:6467321
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g194900 CYA4 Lipocalin-like adenylate cyclase; (1 of 3) PTHR11017//PTHR11017:SF145//PTHR12203//PTHR12203:SF13 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED // KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // F10K1.7 PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACAGTGAGTGATTCGCAGCCGGCGAATG
Internal bar code:CAACACCGGTCAGCGTTGTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:883
LEAP-Seq percent confirming:99.2509
LEAP-Seq n confirming:3975
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACTGACGACGAATCGCT
Suggested primer 2:TCTCTGCAGCTGTATGGTGG