Insertion junction: LMJ.RY0402.061488_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.061488
Chromosome:chromosome 12
Location:9563452
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g547302 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTGATGTGTACGGCATGCATGCAAAAG
Internal bar code:CTCGCGGTGTTTGGACCACCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:99.1105
LEAP-Seq n confirming:780
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTTCTCCTGCACCAGTCC
Suggested primer 2:CCTGGTGGCCTGAACTATGT