| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061492 |
| Chromosome: | chromosome 16 |
| Location: | 4904978 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAGCAGTCTGATTGCGAGCGTCAAGGC |
| Internal bar code: | TATTATTGGTCTTAGCGAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 98.8947 |
| LEAP-Seq n confirming: | 5458 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCCACACTCCCAGTACA |
| Suggested primer 2: | CTGGGTGATCTCGGTACGTT |