| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061506 |
| Chromosome: | chromosome 7 |
| Location: | 3504358 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335950 | (1 of 2) K10523 - speckle-type POZ protein (SPOP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACCGCAGTCTGCCGGTTCAGCATTGCG |
| Internal bar code: | ACGCCATGTGGCTACAATCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 99.8462 |
| LEAP-Seq n confirming: | 649 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGGGAGATGAAGCATCG |
| Suggested primer 2: | GGATGTTTTGTTTGTGCGTG |