| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061511 |
| Chromosome: | chromosome 12 |
| Location: | 6464300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538200 | (1 of 1) PTHR23305//PTHR23305:SF1 - GTP-BINDING PROTEIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGCACGGCGAGAACGTATTGGTGGAA |
| Internal bar code: | ACGGTCGCACGGCAGCTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 97.3595 |
| LEAP-Seq n confirming: | 1143 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGAAGGACTCGGCGTAG |
| Suggested primer 2: | CATGGTCCAACACAAACTGC |