| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061522 |
| Chromosome: | chromosome 1 |
| Location: | 5081522 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035300 | CPA2 | (1 of 2) 3.5.1.53 - N-carbamoylputrescine amidase; N-carbamoylputrescine amidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCACTTTTTCCCTCCGCCATCTGAGCA |
| Internal bar code: | CGGGGCTAATAGGGGGCTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 99.093 |
| LEAP-Seq n confirming: | 2950 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCGCTTGCAAGGGTACT |
| Suggested primer 2: | CTGGGACTCTTCTGCCTTTG |