| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061535 |
| Chromosome: | chromosome 12 |
| Location: | 8612031 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547150 | (1 of 1) 3.5.3.18 - Dimethylargininase / N(G),N(G)-dimethylarginine dimethylaminohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGTAGCGATGGCGTGGAGGAGGGTGG |
| Internal bar code: | CGAAGGTACGGGCCAGAGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 93.5829 |
| LEAP-Seq n confirming: | 525 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGATTCAGTGTCAAGCC |
| Suggested primer 2: | CCAAGCAGTCGCATACTCAA |