| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061543 |
| Chromosome: | chromosome 9 |
| Location: | 6657129 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408800 | (1 of 12) PF04749 - PLAC8 family (PLAC8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCCCCCAGCCTCTTACGCCTCACGCCC |
| Internal bar code: | CCCACCGGCTGCAGCATTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 67.1721 |
| LEAP-Seq n confirming: | 2392 |
| LEAP-Seq n nonconfirming: | 1169 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGAAAGCACGTAAGCGA |
| Suggested primer 2: | CAGAAAGGTAGCCTGGCTTG |