Insertion junction: LMJ.RY0402.061607_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.061607
Chromosome:chromosome 2
Location:5108615
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g104951 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCATTTCAAGACATGAGGTGGGACCAG
Internal bar code:AACCCCTGCGCGTGCTATTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:849
LEAP-Seq percent confirming:99.6444
LEAP-Seq n confirming:1401
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCAGTGAGTGGGGGTTGT
Suggested primer 2:AGCCAACTCCTCCTCCAGTT