| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061655 |
| Chromosome: | chromosome 12 |
| Location: | 6375934 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537581 | (1 of 1) 3.5.4.10 - IMP cyclohydrolase / Inosinicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCAGATGGAAATGGCCCCGGTTGTGC |
| Internal bar code: | AAAGTCAGGGTGGGTGGACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.7484 |
| LEAP-Seq n confirming: | 793 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCACGTCAAGTCCAAC |
| Suggested primer 2: | CATTGACGTTCATGTCTGGG |