| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061658 |
| Chromosome: | chromosome 17 |
| Location: | 1196497 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704950 | (1 of 2) PTHR13140//PTHR13140:SF410 - MYOSIN // SUBFAMILY NOT NAMED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGAAGCGCGGCGTGAGTGAAGCCGGGT |
| Internal bar code: | GACTGTCAAGGGAGGAGGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 99.2147 |
| LEAP-Seq n confirming: | 1137 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGTCTGTGCATAGGGA |
| Suggested primer 2: | CTTAGGGACAAGGCTGCAAG |