| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061660 |
| Chromosome: | chromosome 12 |
| Location: | 452149 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493550 | THK3 | Thymidine kinase; (1 of 1) PTHR11652//PTHR11652:SF1 - 30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER // 28S RIBOSOMAL PROTEIN S12, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGTGACGGCGTTGACCATTGCCTTATGG |
| Internal bar code: | TGTGGCGCACACTGGAGTGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 97.6805 |
| LEAP-Seq n confirming: | 2611 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGTGCGT |
| Suggested primer 2: | ATGCATACGGTGTGGTGCTA |