| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061670 |
| Chromosome: | chromosome 12 |
| Location: | 3265075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGCGGAATGCTGGCGCGAATGCGAGGC |
| Internal bar code: | TCTCCTTACTCTTCAGCTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 99.004 |
| LEAP-Seq n confirming: | 497 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTACCACAGGAATCTGA |
| Suggested primer 2: | CCTCACCCTAGCTTGTCAGC |