Insertion junction: LMJ.RY0402.061679_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.061679
Chromosome:chromosome 6
Location:6792339
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g295001 (1 of 100) IPR001810 - F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGATTGCCTACGCCTACCATTTCCTTAAC
Internal bar code:GACGGCGCTTCTCCGTAGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:140
LEAP-Seq percent confirming:98.2759
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACACACACCTCTTTCCT
Suggested primer 2:AACGTCACCACACCAGTTGA