| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061686 |
| Chromosome: | chromosome 17 |
| Location: | 5427927 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTTGCTGCGGCCGCGTCGGGCACGTGC |
| Internal bar code: | AGGCTTTATATGTAGGCCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.5728 |
| LEAP-Seq n confirming: | 10723 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGTACGCAAGGTGTGT |
| Suggested primer 2: | GGTGGATGTTCCTGACGACT |