| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061760 |
| Chromosome: | chromosome 1 |
| Location: | 3090106 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019200 | NOP52,GEX18 | RRP1-like protein; (1 of 1) K14849 - ribosomal RNA-processing protein 1 (RRP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAATTGTTCTGGAACGACGACATGACG |
| Internal bar code: | GTCGTAGGAAGTGGTGACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 99.4889 |
| LEAP-Seq n confirming: | 584 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTTCAGCTTGGTCAACA |
| Suggested primer 2: | TTCGCTTGCTAGGTGTGATG |