Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.061762 |
Chromosome: | chromosome 4 |
Location: | 1239578 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g215200 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCGCACGAGTGTCATACCACGATGTCA |
Internal bar code: | AACAGGCAGGGCAAGACGGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 732 |
LEAP-Seq percent confirming: | 99.3167 |
LEAP-Seq n confirming: | 4215 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGTTTCAAAGCTTGTCGCC |
Suggested primer 2: | ACCTGCATTCAGTGGGTAGG |