| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061766 |
| Chromosome: | chromosome 16 |
| Location: | 2342470 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659600 | (1 of 1) K14834 - nucleolar complex protein 3 (NOC3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTCGGAAGAGCAGGACGGGCAGGCAGG |
| Internal bar code: | ATGGGCGATCGTCCTACATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 257 |
| LEAP-Seq percent confirming: | 13.0723 |
| LEAP-Seq n confirming: | 474 |
| LEAP-Seq n nonconfirming: | 3152 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGGTGTGGGTTTGCCAG |
| Suggested primer 2: | GGACAACGTGACCGCTAAAT |