| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061768 |
| Chromosome: | chromosome 6 |
| Location: | 5992604 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289050 | (1 of 33) IPR013763 - Cyclin-like | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCTCGGAGCGGCTCTGGGAACAATGCT |
| Internal bar code: | ATTACTATTTCAGCTTCATAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 93.5294 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGGTTGATGTTCCGAG |
| Suggested primer 2: | GAGTTTTGCTAGGCTCACGG |