Insertion junction: LMJ.RY0402.061773_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.061773
Chromosome:chromosome 9
Location:4140883
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393839 (1 of 21) IPR000679 - Zinc finger, GATA-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGACACGCGACGGGTGTGGCCGCCAGGA
Internal bar code:CGAGCGCCGCGGGCAATCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:96.7236
LEAP-Seq n confirming:2037
LEAP-Seq n nonconfirming:69
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCACAAAGCATCGGAACAAA
Suggested primer 2:CGGACTCGGTCTCCTCTTTA