Insertion junction: LMJ.RY0402.061774_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.061774
Chromosome:chromosome 14
Location:2758546
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g627100 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAAGACGGCTCTGCGCACTGTGAAGAG
Internal bar code:GCGTGCCATCGGATGCTGCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:264
LEAP-Seq percent confirming:99.7346
LEAP-Seq n confirming:1879
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGTCGAGCCTAAATGTGC
Suggested primer 2:CGTTGGTCAGTGTTTGGTTG