| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061794 |
| Chromosome: | chromosome 12 |
| Location: | 4287265 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACCCGGCCGCTTCACGTGGAACCACTG |
| Internal bar code: | ACGTAGCCGGGATTTGAAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 99.5078 |
| LEAP-Seq n confirming: | 2628 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTAGCTGGGCACAATGACC |
| Suggested primer 2: | AGGTCCGCAGATGGTAGTTG |