| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061795 |
| Chromosome: | chromosome 3 |
| Location: | 163647 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144184 | (1 of 10) IPR016185 - Pre-ATP-grasp domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACTCCCATTTCTTCGTACGAGACGACT |
| Internal bar code: | GATAAGCAATACGGACAAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 95.3668 |
| LEAP-Seq n confirming: | 247 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATACGAGGCCAAGTACGGC |
| Suggested primer 2: | ATGCGTCAACCTCTGCTCTT |