| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061797 |
| Chromosome: | chromosome 16 |
| Location: | 1673238 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654600 | PHC56 | (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCAGGGCTGGAAGGCGCCGGGGACGG |
| Internal bar code: | AGTAGGGGGGGGGGTATGAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 89.7959 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAAAATGCAGCAGGCTT |
| Suggested primer 2: | ACCAACTGCAGAGCTTCCAC |