| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061800 |
| Chromosome: | chromosome 2 |
| Location: | 6639182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117400 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGAGAAAGGCGTGGTGCGGAACAGGGCG |
| Internal bar code: | GTACGGTTGATTCGGACGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 99.408 |
| LEAP-Seq n confirming: | 2183 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCCACTGTTGTTGGC |
| Suggested primer 2: | ACTGCATGTATCCAGAGGGG |