| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061804 |
| Chromosome: | chromosome 1 |
| Location: | 5084008 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035300 | CPA2 | (1 of 2) 3.5.1.53 - N-carbamoylputrescine amidase; N-carbamoylputrescine amidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGGGTTGGCGGGGTTGAGGTTGAAA |
| Internal bar code: | CGTTGGTTTTGGTTCCTTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 98.5796 |
| LEAP-Seq n confirming: | 4650 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAGTGTGCTGTGGGAAG |
| Suggested primer 2: | GCACATGACAACGCCAATAC |