| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061809 |
| Chromosome: | chromosome 2 |
| Location: | 3926957 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096400 | NDUFAF7 | (1 of 1) K18164 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 7 (NDUFAF7); NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTGTCAGCTCCCACTCGTGCGCCTGCC |
| Internal bar code: | GCCACTTGATACGATCTACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 43.6364 |
| LEAP-Seq n confirming: | 216 |
| LEAP-Seq n nonconfirming: | 279 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTCGCAGTAGCACACT |
| Suggested primer 2: | GGTACTGGTGGTGGTGCTTT |