| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061826 |
| Chromosome: | chromosome 5 |
| Location: | 2060950 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234550 | ALDCHL,FBA3 | (1 of 1) PTHR11627//PTHR11627:SF8 - FRUCTOSE-BISPHOSPHATE ALDOLASE // FRUCTOSE-BISPHOSPHATE ALDOLASE 3, CHLOROPLASTIC-RELATED; Fructose-1%252C6-bisphosphate aldolase, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCAACACCACACCCACAGCCCGGCCAC |
| Internal bar code: | ACCAGACGTGACCGAAGAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 93.4177 |
| LEAP-Seq n confirming: | 10928 |
| LEAP-Seq n nonconfirming: | 770 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAGACTTTCACGGAAC |
| Suggested primer 2: | CCCTCTATTCACCTCCACCA |