| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061829 |
| Chromosome: | chromosome 1 |
| Location: | 4587285 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCAGGAGGCGGGGGGGGGGGGCAGTCA |
| Internal bar code: | GTGGAGTCCCACGATTCGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 99.2747 |
| LEAP-Seq n confirming: | 3148 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACACCAACGACCTGACC |
| Suggested primer 2: | AAGTGTTGCTGGGTCAGCTT |