| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061833 |
| Chromosome: | chromosome 3 |
| Location: | 8523387 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201327 | AKR1 | Aldo/keto reductase; (1 of 1) 1.1.1.188//1.1.1.283 - Prostaglandin-F synthase / Prostaglandin-F synthetase // Methylglyoxal reductase (NADPH) / Methylglyoxal reductase (NADPH-dependent) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAACACGGAGCCGCGGGGCAGCCCGCTG |
| Internal bar code: | GATTCAGCCGCCCTCCGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCTCTCCACCCTACCC |
| Suggested primer 2: | TTCTGACTTTCGCACCACTG |