| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061834 |
| Chromosome: | chromosome 17 |
| Location: | 438216 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698950 | (1 of 1) K09140 - pre-rRNA-processing protein TSR3 (TSR3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGTATCGCCCGTTATGCCGAACCCAC |
| Internal bar code: | GTGTCGGTACAGCCGTGCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 121 |
| LEAP-Seq percent confirming: | 68.8487 |
| LEAP-Seq n confirming: | 1483 |
| LEAP-Seq n nonconfirming: | 671 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACACTGAACCTCCCCTG |
| Suggested primer 2: | ACACAGCGACCCCTTATCAC |