| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061835 |
| Chromosome: | chromosome 12 |
| Location: | 5907464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534100 | COL,CON2 | (1 of 3) PTHR31319//PTHR31319:SF10 - FAMILY NOT NAMED // ZINC FINGER PROTEIN CONSTANS-RELATED; CONSTANS-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGGGCATCAATTTATTCTGCGCTGCG |
| Internal bar code: | TTGGGAAGGTTGGCAAACGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.515 |
| LEAP-Seq n confirming: | 6771 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGATTAGGACCTGCAATA |
| Suggested primer 2: | CTTGTTCCAGAGAGGGCTTG |