Insertion junction: LMJ.RY0402.061861_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.061861
Chromosome:chromosome 2
Location:285389
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATTCTCTTCTCTATGTCCGCCCAAAATG
Internal bar code:TGAGGGGGGAAGCTGGTGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:885
LEAP-Seq percent confirming:98.3707
LEAP-Seq n confirming:483
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCGCTTGATATTCAGCTT
Suggested primer 2:CGGTACCCTGATGTCGAGTT