Insertion junction: LMJ.RY0402.061881_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.061881
Chromosome:chromosome 17
Location:1636706
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g708038 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTTGTTATTTCTACGTGCGGGGGGCGGC
Internal bar code:AGGGGGGACGATCCGGGCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:488
LEAP-Seq percent confirming:86.5716
LEAP-Seq n confirming:8136
LEAP-Seq n nonconfirming:1262
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACAACGACTGTCCCTTC
Suggested primer 2:CGCCTAACTTGTTGTCACGA