| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061890 |
| Chromosome: | chromosome 3 |
| Location: | 9142185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g211969 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAAACTAAACTGAGAGGGCTGGGAGGG |
| Internal bar code: | CGCACGGATTGGGAAAAAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 15.4037 |
| LEAP-Seq n confirming: | 744 |
| LEAP-Seq n nonconfirming: | 4086 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAACAAGACCAGCAACG |
| Suggested primer 2: | AGGCCCTAGTGCCCTACAAT |