| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061955 |
| Chromosome: | chromosome 7 |
| Location: | 452945 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315432 | CSG3 | Chlamydomonas specific family G protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATAGCTGGACTTGCTACGGCTCCACGT |
| Internal bar code: | AACTAGCGTTAAGTGGGGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 99.0317 |
| LEAP-Seq n confirming: | 5318 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATACCGCACTGGAAATG |
| Suggested primer 2: | GTCACTCCTCGCTCACATCA |