Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.061972 |
Chromosome: | chromosome 9 |
Location: | 1163413 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g399950 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGTGTGTCTCATCACCCGCCCATCCCC |
Internal bar code: | CCGGACGATCTCACATGTGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 210 |
LEAP-Seq percent confirming: | 85.7371 |
LEAP-Seq n confirming: | 1611 |
LEAP-Seq n nonconfirming: | 268 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGGGTTGATACCGTTGAA |
Suggested primer 2: | GATCTCTCTAGCACCCGCAC |