| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061979 |
| Chromosome: | chromosome 5 |
| Location: | 219246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233900 | APX4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTTTGTGCAGAGTGTTCGGGCAAGGTG |
| Internal bar code: | TCTGAACAACGCTCGCGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 99.6262 |
| LEAP-Seq n confirming: | 4531 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGGTACTTCCGATGACTG |
| Suggested primer 2: | CTTCCCCCATCTGCTTATCA |