| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062086 |
| Chromosome: | chromosome 6 |
| Location: | 1142102 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257050 | GBS2 | Granule bound starch synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGACTTGGCCGGAGCACGAGTTGGCGA |
| Internal bar code: | CCACAACTGTACACCCCAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 97.8571 |
| LEAP-Seq n confirming: | 8220 |
| LEAP-Seq n nonconfirming: | 180 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGCTTAGCTTTAGGCAA |
| Suggested primer 2: | TGTCAATCACAAGCACAGCA |